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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Intratumoral Distribution of Lactate and the Monocarboxylate Transporters 1 and 4 in Human Glioblastoma Multiforme and Their Relationships to Tumor Progression-Associated Markers
doi: 10.3390/ijms21176254
Figure Lengend Snippet: Expression of MCT (monocarboxylate transporter) 1, MCT4, EMT (epithelial to mesenchymal transition) markers (vimentin, β-catenin), stem-like cell markers [KLF4 (krüppel-like factor 4), OCT4 (octamer-binding transcription factor 4), Sox2 (sex-determining region Y-box 2)], dormancy markers [EPHA5 (ephrin receptor A5), H2BK (histone cluster 1 H2B family member k), IGFBP5 (insulin-like growth factor-binding protein 5)] and GFAP (glial acidic fibrillary protein) in pairs of center and edge glioblastoma samples was evaluated by quantitative reverse transcription PCR (logarithmic scale, a Δ C t = 3.33 increase corresponds to a 10-fold decrease in gene expression). ( a ) Shows the n-fold changes of each marker between the center and the edge of the tumor (calculated with the average values of 2 −( Δ C t edge − Δ C t center ) = n-fold), small dots plot the n-fold value of each probe, whereas ( b ) plots the Δ C t values itself. GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and TBP (TATA-box-binding protein) served as internal standards. The expression of MCT1 was significantly lower (* p : 0.025), whereas GFAP (* p : 0.010), β-catenin (* p : 0.039), KLF4 (* p : 0.035) and OCT4 (* p : 0.026) showed a significant higher expression at the edge of the tumor compared with the center, as determined by two-tailed Student’s t -test. Furthermore, a slightly increased expression (more than 2-fold) of EPHA5 and IGFBP5 was detectable at the edge of the tumor compared with the center.
Article Snippet: In combination with anti-MCT1 (1:500, mouse, sc-365501, Santa Cruz Biotechnology Inc., Dallas, TX, USA) and anti-MCT4 (1:150, mouse, sc-376101, Santa Cruz Biotechnology Inc.), the cells were stained with the specific EMT antibodies β-catenin (1:150, mouse, 651109, BD Transduction Laboratories, Franklin Lakes, NJ, USA) and vimentin (1:50, mouse, sc-6260, Santa Cruz Biotechnology Inc.), as well as the stem cell antibodies KLF4 (1:250, mouse, MA5-15672, Thermo Fisher Scientific), OCT4 (1:150, rabbit, #2750, Cell Signaling, Danvers, Massachusetts, USA), Sox2 (1:200, rabbit, sc-20088, Santa Cruz Biotechnology Inc.), the
Techniques: Expressing, Binding Assay, Reverse Transcription, Gene Expression, Marker, Two Tailed Test
Journal: International Journal of Molecular Sciences
Article Title: Intratumoral Distribution of Lactate and the Monocarboxylate Transporters 1 and 4 in Human Glioblastoma Multiforme and Their Relationships to Tumor Progression-Associated Markers
doi: 10.3390/ijms21176254
Figure Lengend Snippet: Representative co-staining of MCT1 with EMT markers (β-catenin, vimentin), stem-like cell markers (KLF4, OCT4, Sox2), dormancy markers (EPHA5, H2BK, IGFBP5) and GFAP, as well as vWF in pairs of center and edge glioblastoma samples as determined by immunofluorescence microscopy. MCT1 and MCT4 (green) are found in different combinations with cell-specific markers (red) in a complex pattern. Magnification 400×; bar = 20 μm.
Article Snippet: In combination with anti-MCT1 (1:500, mouse, sc-365501, Santa Cruz Biotechnology Inc., Dallas, TX, USA) and anti-MCT4 (1:150, mouse, sc-376101, Santa Cruz Biotechnology Inc.), the cells were stained with the specific EMT antibodies β-catenin (1:150, mouse, 651109, BD Transduction Laboratories, Franklin Lakes, NJ, USA) and vimentin (1:50, mouse, sc-6260, Santa Cruz Biotechnology Inc.), as well as the stem cell antibodies KLF4 (1:250, mouse, MA5-15672, Thermo Fisher Scientific), OCT4 (1:150, rabbit, #2750, Cell Signaling, Danvers, Massachusetts, USA), Sox2 (1:200, rabbit, sc-20088, Santa Cruz Biotechnology Inc.), the
Techniques: Staining, Immunofluorescence, Microscopy
Journal: International Journal of Molecular Sciences
Article Title: Intratumoral Distribution of Lactate and the Monocarboxylate Transporters 1 and 4 in Human Glioblastoma Multiforme and Their Relationships to Tumor Progression-Associated Markers
doi: 10.3390/ijms21176254
Figure Lengend Snippet: Representative co-staining of MCT4 with EMT markers (β-catenin, vimentin), stem-like cell markers (KLF4, OCT4, Sox2), dormancy markers (EPHA5, H2BK, IGFBP5) and GFAP, as well as vWF, in pairs of center and edge glioblastoma samples, as determined by immunofluorescence microscopy. MCT1 and MCT4 (green) are found in different combinations with cell-specific markers (red) in a complex pattern. Magnification 400×; bar = 20 μm.
Article Snippet: In combination with anti-MCT1 (1:500, mouse, sc-365501, Santa Cruz Biotechnology Inc., Dallas, TX, USA) and anti-MCT4 (1:150, mouse, sc-376101, Santa Cruz Biotechnology Inc.), the cells were stained with the specific EMT antibodies β-catenin (1:150, mouse, 651109, BD Transduction Laboratories, Franklin Lakes, NJ, USA) and vimentin (1:50, mouse, sc-6260, Santa Cruz Biotechnology Inc.), as well as the stem cell antibodies KLF4 (1:250, mouse, MA5-15672, Thermo Fisher Scientific), OCT4 (1:150, rabbit, #2750, Cell Signaling, Danvers, Massachusetts, USA), Sox2 (1:200, rabbit, sc-20088, Santa Cruz Biotechnology Inc.), the
Techniques: Staining, Immunofluorescence, Microscopy